Biography:
Raphael Gorodetsky is the Head of Laboratory of Biotechnology and Radiobiology at the Sharett Institute of Oncology, Hadassah Medical Center, affiliated to the Hebrew University in Jerusalem. He did his PhD at the Hebrew University in 1985 and Post-Doc at UCLA Medical Center. His earlier research interests were in developing new technologies for in-vivo analyses of trace elements. His interests include regenerative radiobiology and biomaterials for regenerative medicine with fibrin based biomaterials and interactions with different cell types. In applied research, he was the Founder and the Chief Scientist of Hapto Biotech, later merged to form Forticel International SA and was involved in numerous other ventures. Recently, he developed placental stromal cells based therapy for modulating regenerative processes and mitigation of radiation effects. Besides his numerous publications, he co-edited the book “Stem Cells Tissue Repair” (RSC publishers, Cambridge, UK). He is the Secretary of Israel Stem Cells Society (2014-2017) and is Member in different international societies.
Abstract:
Based on the haptotactic (
cell binding) properties of fibrin, we have conducted extensive studies on the cell binding properties of fibrinogen and developed new Fibrin-based dense dehyrothermally crosslinked biodegradable Microbeads (FMB) for culturing high density of cells in suspension for their implantation. The FMB can attract and carry high number of matrix dependent cells of different types and can be used to culture stromal cells of different origin including fibroblasts, placental cells, endothelial cells, smooth muscle cells, chondrocytes and osteoblasts as well as endothelial cells. We employed FMB to isolate mesenchymal pluripotent
stem cells from bone marrow and cord blood to expand them in vitro, and to induce differentiation in vitro or to implant as progenitors cells to produce cartilage and bone tissues. In parallel studies on the mechanism of cell binding to fibrin we identified new family of ~20mer epitopes on fibrinogen that seem to be responsible for the selective haptotactic activity of fibrin based matrices. Synthetic peptides of these sequences on a matrix could be used to augment cell binding to matrices in
tissue engineering. The family of the short haptotactic peptidic sequences, which were termed haptides were also investigated as potent cell penetrating agents following fibrinolysis, as well as non-immunogenic synthetic cell penetrating sequences. The possible systemic adverse effects of penetrating peptides as well as fibrin derived haptides were also investigated in parallel.
Recent Publications:
1. Raphael Gorodetsky, Shainer R, Gaberman E, Levdansky L and Gorodetsky R (2010) Efficient isolation and chondrogenic differentiation of adult mesenchymal stem cells with fibrin microbeads and micronized collagen sponges. Regen. Med. 5(2): 255-65.
2. Raphael Gorodetsky, Levy-Beladev L, Levdansky L, Gaberman E, Benyamini H and Friedler A (2010) A family of cell adhesion peptides homologous to fibrinogen C-terminals. Biochemical and Biophysical Research Communications 401: 124-130.
3. Raphael Gorodetsky, Basheer, M Schwalb H, Nesher M, Gilon D Shefler I, Mekori, J A Shara O M (2010) Mast cell activation by fibrinogen-related homologous c-terminal peptides (haptides) modulates systemic blood pressure. J Allergy Clin Immunol 126:1041-1048.
4. Raphael Gorodetsky, Basheer M, Schwalb H, Shefler I, Levdansky L T, Mekori JA, Gorodetsky R (2011) Blood pressure modulation following activation of mast cells by cationic cell penetrating peptides. Peptides 32 (12): 2444-2451.
5. Raphael Gorodetsky, Xie M W, Gorodetsky Ro, Micevicz E, Mackenzie N C, Gaberman E, Levdansky L and McBride W Ho (2013) Marrow stromal cells delivery on fibrin microbeads can correct radiation-induced wound healing deficits. Journal of Investigative Dermatology 133 (2): 553-561.
