Jozsef Bako
University of Debrecen, Hungary
Title: Biodegradable polymer based electrospun nanofibers for dental applications
Biography
Biography: Jozsef Bako
Abstract
Electrospinning is a versatile method for fabrication of submicron sized fibers from biopolymers, ceramics and composite materials. The dental application possibilities of these nanofibers are intensively research areas on the fields of tooth or pulp regenerations, prevention of dental caries, or drug delivery systems. Biopolymers can facilitate the elasticity of created structures, and ensure the similarities to the extracellular matrix. The tailoring of the diameters of the fibers, and pore sizes of the structures ensures the optimal conditions for the proliferation and differentiation of cells. The delivery of biological active ingredients, factors, or drugs can achieve fast and supported regeneration. Composite materials give possibly of adjusting physical, biological, or release properties. Nanofibers combined with inorganic ceramics, or polymers with nanoparticles can create functional materials for the speed up wound healing, or osseointegration processes.
Polymer solutions were created for electrospinning process from Poly-vinyl-alcohol (18-88 Ph, Eur.Merc) (PVA) in 10 w/w% and 50% precrosslinked, and 50% methacrylated-poly-γ-glutamic acid nanoparticles (MPGA-NP) in 15w/w%. Irgacure 2959 1w/w% was added to the composite as photoinitiator. Nanofiber fabrication was performed by Nanospinner NS1 electrospinning device (Inovenso). The created fiber matts were phtopolymerized by Bluehase 20i (Ivoclar Vivadent) dental lamp for 60sec, and the biocompatibility properties was investigated by SAOS-2.
The diameters of created MPGA/PVA nanofibers were between 82.1-149.2 nm with the average of 120.7±17.5 nm. The crosslinking of the fibers by post photopolymerization was successful, and the fibers not dissolved during the one week cell proliferating test. The good biocompatibility of the created electrospun nanofibers was proved by the presence and proliferation of SAOS cells.
This nanoparticles in the nanofibers construction allow and enhance the control of different drug releases. The work is supported by the GINOP-2.3.2-15--2016-00011 and GINOP-2.3.2-15-2016-00022.. project. The project is co-financed by the European Union and the European Regional Development Fund.