Day 1 :
Keynote Forum
Gianfranco Peluso
IRECT-CNR, Italy
Keynote: Cell/biomaterials interaction
Time : 10:30-11:10
Biography:
Prof. Gianfranco Peluso graduated magna cum laude and special mention in Medicine at the University of Naples, Italy. He has been Director of the Department of Experimental Oncology at the National Cancer Institute. Currently, he is Research Director at Italian National Research Council. His scientific activity and areas of interest include: nanoscience and nanotechnology applied to biomedicine, life science and food security, for: a) development of nanostructured polymers as novel delivery platform to minimize drug degradation upon administration, prevent undesirable side effects, and sustain and/or increase drug's bioavailability in a targeted area, and b) synthesis of biodegradable polymers for innovative food packaging to improve shelf life, microbiological safety and sensory properties of foods without affecting their organoleptic and nutritional characteristics.
Abstract:
The cell/material interaction is a complex, dynamic process in which the cell and the material synergistically influence the fate of the cell. Indeed, both materials intrinsic (i.e. topography, charge, ζ-potential, and contact angle) and extrinsic properties (i.e. surface functionalization, crystallinity, etc.) played a pivotal role in dictating the type and strength of the biological responses (Figure 1). Furthermore, the ability of biomaterials to release bioactive molecules (i.e. resveratrol, fluoride, etc.) expands the possibilities to control cell-cell interactions and/or intracellular signal transduction. Our recent research demonstrated a functional role of charged polymers in altering or supporting the osteogenic differentiation of mesenchymal stem cells (MSCs) through the modulation of the ephrinB2/EphB4 interaction. Indeed, cell-cell signaling pathways that lead to efficient differentiation of stem cells include the interaction of Ephrin ligands (ephrinB2) with Eph receptors (EphB4). For the first time we have shown that high charged polymers can affect the Eph/ephrin interaction between neighboring cells inhibiting the MSCs osteogenic differentiation via the perturbation of the bidirectional signaling. In contrast, low charged polymers modulate the differentiation of MSCs into an osteocyte lineage via cell-cell ephrinB2/EphB4 signaling.
Keynote Forum
Valentina Cauda
Polytechnic University of Turin, Italy
Keynote: Hybrid smart nanocrystals and the shielding effect of phospholipid bilayer for biomedical application
Time : 11:25-12:05
Biography:
Valentina Cauda pursued her PhD in Material Science and Technology (2008) from Polytechnic University of Torino, Italy. She is graduated in Chemical Engineering (2004). She is currently an Associate Professor at the Polytechnic University of Torino. In 2006, she was a visiting PhD student at the Complutense University of Madrid (Spain). From 2008 to 2010, she worked as Postdoc at the University of Munich (Germany). From 2010 to 2015 she was a Senior Postdoc at the Istituto Italiano di Tecnologia (Italy). For her research work she received several prizes: Young Researchers at the University of Munich (2010); the Italian “Giovedì Scienza” award (2013); Zonta Prize for Chemistry (2015) and the USERN Prize for Biological Sciences (2017) respectively. In 2016 she was awarded by the European Research Council with an ERC Starting Grant. She is the author of 84 papers in peer-reviewed international journals with H-index of 28
Abstract:
Statement of the Problem: Zinc oxide nanocrystals (ZnO NCs), thanks to their unique properties, are receiving much attention for their use in nanomedicine, in particular for therapy against cancer. To be efficiently employed as diagnostic and therapeutic (yet theranostic) tools, highly dispersed, stable and non-toxic nanoparticles are required. In the case of ZnO NCs, there is still a lack of knowledge about cytotoxicity mechanisms and stability in the biological context, as well as immunological response and hemocompatible features. We thus propose a novel approach to render stable, immune and hemocompatible ZnO NCs in various biological media using artificial and natural phospholipidic bilayers.
Methodology & Theoretical Orientation: We synthesized amino functionalized ZnO NCs, then shielded with phospholipid bilayers either from synthetic origin or natural biovesicles. We characterized their structural, morphological, physico-chemical properties, focusing on the coupling mechanism between ZnO NCs and the lipid vesicles. The stability behavior of different hybrid nanocrystals was evaluated, comparing their biodegradation profiles in different inorganic and biological media. The study aims to investigate how the particle surface chemistry and charge could influence their aggregation/degradation in the different media and interaction with cells. We actually proved their hemocompatibility in human plasma and their internalization into cancer cells and related cytotoxicity mechanisms. A stimuli responsive activation by UV-light was investigated for inducing high mortality of cancer cells based on the hybrid NCs.
Findings: We demonstrated that pristine ZnO NCs strongly aggregate when suspended in both simulated and biological media, showing small dissolution into potentially cytotoxic Zn-cations, also slightly affecting their crystalline structure. In contrast, high colloidal stability and integrity was retained for hybrid lipid-shielded ZnO NCs in all media, accompanied by high biocompatibility, efficient cell internalization and effective killing ability only upon stimuli-activation. These features render these hybrid ZnO NCs ideal “Trojan horses” for further theranostic applications.
Keynote Forum
Tofik M Nagiev
Nagiev Institute of Catalysis and Inorganic Chemistry, Azerbaijan
Keynote: New approaches to simulation of enzymatic reactions: mimetic catalysis
Time : 12:05-12:45
Biography:
Tofik Nagiev is a Vice-president of Azerbaijan National Academy of Sciences, Director of Research Center of “Azerbaijan National Encyclopedia” and Department Chief of Nagiev Institute of Catalysis and Inorganic Chemistry of ANAS. He is a Professor of the Department of the physical and colloid chemistry of Baku State University.
Abstract:
The area between enzymatic and chemical catalyses, associated with simulation of biochemical processes by their basic parameters, is accepted as mimetic catalysis. The key aspect of mimetic catalyst is diversity of enzyme and biomimetic function processes, which principally distinguishes the mimetic model from traditional full simulation. Basing on the analysis of conformities and diversities of enzymatic and chemical catalysis the general aspects of mimetic catalysis are discussed. Idealized model of biomimetic catalyst and the exclusive role of the membrane in its structural organization are considered. The most important achievements in the branch of catalysis are shown, in particular, new approaches to synthesis and study of biomimetic catalase, peroxidase and monooxidases reactions. The catalysis direction, originated from simulation of biochemical processes, is suggested to call the ‘mimetic catalysis’. Mimetic catalysis designs a real model (a mimic) which simulates objects and processes of enzymatic catalysis by their basic (but deficient) characteristics (selectivity, condition mildness, active site action mechanism etc.). Since only definite properties of enzyme are simulated, it does not pretend to completeness of enzyme description, though optimal parameters by some properties may be approached. The mimetic model of enzyme helps in synthesizing suitable catalysts using inaccurate and sometimes ambiguous information. The overwhelming majority of biomimetics operate in liquid. Their activity depends on the origin of solvents, reaction mixture and cell effects. Gas phase oxidation processes are less dependent on these effects and in the first approximation can be considered as oxidation under quasi-ideal conditions. It goes without saying that enzymatic reactions do not proceed in gases.
However, it is possible to simulate catalytic functions in the gas phase. However, it is possible to simulate catalytic functions in the gas phase. This simplifies the decoding of the reaction mechanism, not complicated by factors accompanying the liquid-phase oxidation.
- Advanced Bio Materials| Bio Nano materials | Polymer Biomaterials
Location: Wright
Chair
Mehmet Bayrak
Ortadogu Private Hospital, Turkey
Co-Chair
P V Rao
IIT Delhi, India
Session Introduction
Liana Azizova
University of Brighton, UK
Title: Argatroban immobilization on Cu-modified PVC and PU
Biography:
Liana Azizova received her Master degree at Taurida National V.I. Vernadsky University in 2005. She got PhD in Surface Chemistry in 2013. She has her expertise in the area of surface chemistry, surface characterization, catalytic reactions on the surface of inorganic oxides, their kinetics and mechanisms and synthesis of hybrid organicinorganic nanocomposites. It includes the development of biocompatible coating materials and composites for biomedical application. In particular, modification of polymer and nanosized oxides surfaces by biologically active compounds and biopolymers (polysaccharides, glycopeptides, carboxylic acids), adsorption and determination of a structure of an adsorption layer of biomolecules on a surface of inorganic oxides. Also, she dealt with heterogeneous catalytic reactions on the surface of inorganic oxides, their kinetics and mechanisms. Another area of her activity is mass spectrometric investigation of biomolecules interaction with inorganic oxide surfaces and thermal transformations of biomolecules on inorganic oxide surfaces using thermal analysis.
Abstract:
Thrombosis induced by biomaterials after their contact with blood is a main reason of medical device failure. To make material surface more thromboresistant different approaches have been undertaken. NO generating biomaterial has proven to play a crucial role in the prevention of thrombosis by inhibiting the platelets activation/adhesion. However, immobilization of the direct thrombin inhibitors onto material surface makes material more thromboresistant by preventing thrombin-mediated blood clotting. The aim of this research was to immobilize argatroban a direct thrombin inhibitor with reliable and predictable anticoagulant effect onto PVC and PU polymers. Both polymers were first imprinted with Cu ions for the catalytic generation of NO (this research was reported earlier). Argatroban was immobilized on the Cu modified PVC and PU using the polydopamine ad-layer via the Michael addition/Schiff base reaction. The amount of argatroban bound to the polymer surface was measured (spectrophotometric determination at 334 nm) as 11.92 nmol/cm2 on PVC and 13.10 nmol/ cm2 on PU surface. Assay using thrombin specific chromogenic substrate was performed to evaluate the thrombin inhibition capacity of argatroban-modified polymers. It was found that both Argatroban-modified polymers inhibit thrombin activity in PBS. In order to confirm the NO generation catalyzed by Cu/Arg-modified PVC and PU samples after incubation with 100 μM GSNO/GSH in the PBS during 1h was examined using ArrowSTRAIGHT™ nitric oxide measurement system (Lazar Research Laboratories, Los Angeles, CA, USA). The Cu/Arg-modified PVC and PU generate NO with the rate 1.27-1.66×1010 mole/cm2∙min which is within the physiological level. From the data obtained it’s possible to conclude, that immobilization of Argatroban to the Cu-modified polymers showed combine abilities: i) generate NO caused by Cu ions and ii) have capacity to inhibit thrombin formed in the blood via surface immobilized argatroban
Biography:
Chara Simitzi graduated as a Chemical Engineer from the National Technical University of Athens (Greece) and then continued her studies in biomedical and tissue engineering. She pursued her MSc in Biomedical Engineering from RWTH Aachen University (Germany); PhD in Biology from University of Crete (Greece). After her PhD she worked at the Foundation for Research and Technology - Hellas Institute in Crete and the Queen Mary University of London (UK) respectively. She is currently a Postdoctoral Research Associate in the group of Professor Day (Applied Biomedical Engineering group) at the University College London. Her scientific interests focus on the cell biomaterial interface and more specifically on the development of novel types of scaffolds for tissue engineering applications and cell culture platforms for in vitro studies to address cell biology questions.
Abstract:
Statement of the Problem: Mesenchymal stem cells (MSCs) are becoming increasingly important due to the broad spectrum of trophic and immunomodulatory factors they secrete. The MSC secretome plays a role in angiogenesis and revascularization, immune modulation and tissue repair; however, there is a lack of methods suitable for controlling this effect. Evidence exists to show cell substrates influence MSC behaviour. Therefore, manipulating the cell substrate could provide improved methods for controlling the secretome for new therapies but there is currently a lack of cell substrates suitable for implantation.
Methodology & Theoretical Orientation: The effect of implantable substrates consisting of biodegradable microparticles with hierarchical topographical features was investigated on MSC behaviour and secretome. Poly(DL-lactide-co-glycolide) microparticles were fabricated via the thermally-induced phase separation technique (TIPS). Three different polymer compositions of lactide/glycolide were studied. Microparticles were characterized in terms of surface topography and porosity. Human adipose-derived MSCs (ADMSCs) were attached to the surface of the microparticles
and cultured for 16 days in xeno-free medium. Cell growth on the microparticles was evaluated at different time-points and compared with cells cultured on tissue culture plastic. The angiogenic activity of the ADMSC secretome was evaluated by ELISA and in vitro angiogenesis assays.
Findings: Three different types of TIPS microparticles with different morphological and physicochemical characteristics were investigated. ADMSCs adhered and proliferated on all types of the microparticles. Vascular endothelial growth factor (VEGF) secretion was increased from cells cultured on the microparticles compared with cells cultured on tissue culture plastic. MSCs attached to microparticles remained viable after 16 days, were capable of migrating from the microparticles, and retained their lineage plasticity.
Conclusion & Significance: Our results show that attaching MSCs to biodegradable TIPS microparticles can influence their growth and secretion of pro-angiogenic growth factor. This finding may provide a new method for regenerative medicine
P.V. Rao
IIT Delhi, India
Title: Experimental investigation of microtopography patterns on bacterial attachment
Biography:
Dr. Venkateswara Rao Paruchuri is currently a Professor & Head, Department of Mechanical Engineering, Indian Institute of Technology Delhi. He received Masters & Doctorate Degrees from Indian Institute of Technology Madras. Prior to that, he obtained Bachelor’s Degree from Regional Engineering College Warangal. He has been faculty at IIT Delhi since 1996. His research interests include Material Removal Processing, Micro/Nano Manufacturing, and Sustainable Machining. He received BOYSCAST fellowship award from Government of India in 1998. He was a visiting Assistant Professor at Oklahoma State University, USA in 1999. He was visiting Professor at Tokyo Institute of Technology, Japan in 2012. He published more than 193 research papers in National and International Journals and Conferences. He supervised 21 Ph.D. and several M.Tech theses. He is also a member of Editorial Board of International Journal of Industrial Engineering: Theory, Applications & Practice. He has undertaken several sponsored research projects as Principal Investigator.
Abstract:
Statement of the Problem: The adhesion of bacterial cells on any surface is the first step of biofilm formation. Biomedical field is more concern about biofilm formation as medical devices get frequently contaminated with bacteria and leading to the formation of mature biofilm which can put patient lives at risk. Researchers have reported that the surface topography with micro patterns affects the initial adhesion of bacterial cells. However, the effect of the number of corners on the behavior of bacterial cells is yet to be investigated. In this work, the different types of microscale geometrical features (Triangle, Square,
Star pattern with four and five corners) are fabricated on polydimethylsiloxane (PDMS) using soft lithography technique. Through static droplet contact angle analysis, it is confirmed that the fabricated micro patterns alter the wettability of plain PDMS surface. The effect of number of corners on the migration and
initial attachment of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) are investigated using fluorescence microscopy. The adhesion of bacterium depends on the number of corners present in the geometrical feature.
Moreover, the bacterial adhesion is correlated with the ratio of pillar cross-sectional area to its perimeter (λ). The experimental investigation can be useful in designing the effective geometry which can be used in making anti biofouling structured polymeric devices for biomedical applications.
Sudarsan Ghosh
IIT Delhi, India
Title: An approach towards improvement of machinability of metallic bio materials
Biography:
Abstract:
With the invention of new technologies and development of the new materials, wide ranges of materials are made available for their usage in medical applications. These materials are referred as biomaterials, among which the metallic biomaterials have become an intensive source of interest for the researchers, doctors and surgeons owing to its costs and highly impressive properties. The metallic biomaterials play a predominant role in dental and orthopedic fields as a structural biomaterial in surgeries. Among all the metallic biomaterials Co-based alloys, Ni-based alloys and Ti-based alloys are gaining more popularity owing to their high mechanical strength and fracture toughness. However, utilizing these materials require high degree of machining. In the current study, an effort has been made towards improving the machinability of Ni-based alloy without making use of any coolant or lubricant during the machining operation keeping in mind the aspect of sustainability. The machinability of the alloy is improved by altering the cutting tool properties and conditions by making use of the mechanical treatment technique i.e. micro abrasive blasting. The process improves the cutting tool performance by altering its surface and sub-surface properties thereby improving the characteristics of machined surface. Dry machining of these metallic biomaterials with the use of altered tools is not only an effort towards sustainability but also helps in reducing any type of reactive action between the two.
Biography:
Indrani Mishra have worked on various techniques like ion irradiation, UV irradiation and plasma etching to modify surfaces of SiOx, TiO2 and PDMS in order to enhance its properties for application as biosurfaces and biosensors. The modified surfaces were interacted with plasmid and branched DNA, also interaction of fibroblast cells with plasma modified surfaces has been studied. She has been working with X-Ray Photoelectron Spectroscopy (VG SYSTEM) and Multimode Atomic force Microscopy (from Bruker with a Nanoscope V controller). She has experience of operation and data analysis of XPS and AFM and contact angle measurements. Apart from this she is acquainted with operation and data analysis of Raman, UV visible, pl and XRD systems. She wish to explore the interaction of biomolecules with nanoporous materials, as these materials due to their large surface area, controlled pore size distribution, controllable pore structure and versatile composition has attracted increasing applications in bioengineering, catalysis and biosensing area.
Abstract:
Experimental results on the behavior of wetting on self-affine surfaces of natively oxidized Silicon (100) are presented. The self-affine surfaces have been prepared by the technique of ion irradiation. These surfaces, as results show, present hierarchical multi-scale rough morphologies, not observed earlier for hydrophilic surfaces. They further demonstrate a wetting behavior which depends on the fractal dimension of the surface after irradiation. Results show that structural properties and fractal dimension of DNA molecules, immobilized on the self-affine surfaces, are effected by the fractal dimension and morphology of the surfaces prior to immobilization. The surfaces are self affine in nature and show hydrophilic behavior. The results presented here show that these surfaces exhibit multi- scale roughness with hierarchical structures. The wetting behavior of water droplets shows a dependence on the
fractal dimension of the surface after irradiation. Results on the Si/SiOx surfaces, after DNA immobilization, are also presented. The surfaces do not demonstrate any hierarchical roughness after immobilization and rather exhibit two dimensional flat-smooth morphology. Modifications in the fractal dimension of DNA on these surfaces are also explored. Present studies can have technological implications for many bio-applications.
Robert Lazeroms
Royal Cosun R&D, The Netherlands
Title: New biobased building blocks for health sciences and biomaterials
Biography:
Abstract:
Cosun biobased products (CBP) offers biobased solutions. The activities range from development to manufacturing and supply of biobased functional chemicals. Within Cosun R&D and CBP, we have developed a biorefinery concept on sugar beet pulp. Within this flexible concept, we are able to isolate cellulosic fibers, and different monosaccharides. One of the key
monosaccharides is D-galacturonic acid. Using mild processing conditions, galacturonic acid can be isolated and selectively oxidized to galactaric acid (commercial name: mucic acid). This molecule has multiple outlets including health sciences. It offers unique functionalities, which are already used in pharmaceutical applications (for example isometheptene mucate). For higher volume outlets, biomaterials can be made using galactaric acid as an intermediate to GalX. This novel platform building block shows new opportunities to polymers and crosslinkers. In shared research with universities, GalX shows good Mw, Tg and E modulus compared to industrial benchmarks. Besides these parameters, the improved water solubility compared to adipic acid is an unmet market need for new formulations.
Mehmet Bayrak
Ortadogu Private Hospital, Turkey
Title: Permacol â„¢ collagen paste injection in anal fistula treatment: retrospective study with eighteen months follow-up
Biography:
Mehmet Bayrak and Yasemin Altıntas have expertise in evaluation and passion in improving the health and wellbeing. Their open and contextual evaluation model based on responsive constructivists creates new pathways for improving healthcare. They have built this treatment model after years of experience in research, evaluation, teaching and administration both in hospital and education institutions. This approach is responsive to all stakeholders and has a different way of focusing.
Abstract:
Introduction: This study aimed to evaluate the applicability, safety, results, and functional performance of Permacol™ collagen paste injection in patients with an anal fistula.
Methods: Thirty-nine patients with anal fistula underwent Permacol™ collagen paste injection between February 2015 and February 2018. The patients were followed up for a total of 18months with intervals of 3, 6, 12 and 18 month recovery conditions monitored. Fifteen patients for a period of 6–8 weeks and two patients for a period of 12 weeks underwent preoperative
insertion of seton.
Results: A transsphincteric anal fistula was present in 20 patients, and an intersphincteric fistula was present in 11 patients. There was a recurrence in 7 patients (17.9%): 1 patient (2.5%) after 1-month follow-up, 3 patients (7.6%) after 3-month follow-up, 2 patients (5.1%) after 6-month follow-up, and 1 patient (2.5%) after 18-month follow-up. A complete recovery was observed in 32(82.0%) patients after a 18-month follow-up. The mean Fecal Incontinence Severity Index (FISI) score was 0.29±0.64 preoperatively and 0.55±1.03 after 12 months.
Conclusion: In this study, we show that Permacol™ is a safe and successful method that does not compromise continence following injection and treatment of patients with an anal fistula.
Sutipat Pairojboriboon
Phramongkutklao Hospital and College of Medicine, Thailand
Title: Used of 3D printing technology in orthopedic oncology: custom surgical guide and patientmatched prosthesis
Biography:
Sutipat Pairojboriboon is an Orthopaedic Surgeon, specialized in bone and soft tissue tumours. His research interest is the 3D printing technology, focusing on the printed models as an assisted tool for surgery and patient-matched prosthesis. He has experience in working with engineers who focus on the 3D printing technology from the Chulalongkorn University, Bangkok, Thailand, 3DMAC in Walter Reed Military Medical Center, USA and materialize medical company for three years. He has designed a lot of custom surgical guides and patient-matched prostheses in order to perform limb sparing surgery. By using the 3D printing technology, he has proved that his works will help many sarcoma patients from amputation.
Abstract:
Statement of the Problem: Presenting the clinical outcome of patient-matched prosthesis and custom surgical guide via 3D printing technology.
Methodology & Theoretical Orientation: Total 11 cases of benign and malignant tumors applying 3D printing assisted surgery which are 6 anatomical models, 11 custom surgical guides, and 4 patient-matched prostheses. Image acquisition was derived from CT scan, 0.5-3 mm slices cut. The contralateral CT scan was used as a prototype for creating the patient-matched
prosthesis while the ipsilateral CT scan was used in anatomical model and surgical cutting guide. Anatomical models, used as the preoperative planning tools, were printed by a fused deposition modeling (FDM) printer with acrylonitrile butadiene styrene (ABS) material and a Binder Jetting machine, 3D Systems ZPrinter 650 using VisiJet PXL materials. 11 custom surgical
guides were printed by Envision TEC’s E-Guide Tint and E-Model. 4 of patient-matched prostheses, which are 2 fingers prostheses, 1 of total constrained proximal interphalangeal (PIP) joint prosthesis, and 1 of navicular 3D custom scaffold, were printed by selective laser-melted (SLM) printer with Ti6Al4V. The pore geometry selective laser-melted Ti6Al4V bone
scaffolds was 200 μm, strut size and 500 μm, pore size. Time to produce was 3-20 days. Preoperative planning via anatomical model showed better outcomes in term of decrease operative time and blood loss. Custom surgical guide demonstrated better outcomes comparing to navigation surgery in term of achieving same accuracy but less resection time. According to bone
tumors can be found in the unusual locations which there is no off the shelf prosthesis, patient-matched prosthesis has gained popularity and played a major role in this area.